Composite

Part:BBa_K3429013:Design

Designed by: Jan Lars Kalkowski   Group: iGEM20_TU_Darmstadt   (2020-10-19)


TasA-EreB fusion protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1206
    Illegal XhoI site found at 1616
    Illegal XhoI site found at 2206
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1333


Design Notes

Optimized for B. subtilis.


Source

synthetic, E. coli, B. subtilis

References